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Series GSE20605 Query DataSets for GSE20605
Status Public on Jul 09, 2010
Title R. microplus expression profiles of larvae pre-attachment and feeding adult female stages in B. indicus and B. taurus
Organism Rhipicephalus microplus
Experiment type Expression profiling by array
Summary Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and B. taurus cattle
Global analysis of gene expression changes in R. microplus during larval, pre-attachment and early adult stages of its life cycle feeding on Bos indicus and Bos taurus cattle were compared using gene expression microarray analysis. Among the 13 601 R. microplus transcripts from BmiGI Version 2 we identified 297 up and 17 down regulated transcripts were differentially expressed between R. microplus feeding on tick resistant cattle [Bos indicus (Brahman)] compared to R. microplus feeding on tick susceptible cattle [Bos taurus (Holstein-Friesian)]. These include genes encoding enzymes involved in primary metabolism, and genes related to stress, defence, cell wall modification, cellular signaling, receptor and cuticle. Microarrays were validated by qRT-PCR analysis of selected transcripts including the validation of three housekeeping genes. The analysis of all tick stages under survey suggested a coordinated regulation of defence proteins, proteases, and protease inhibitors to achieve successful attachment and survival of R. microplus on different host breeds particularly Bos indicus cattle.
 
Overall design The microarray was conducted by NimbleGen Systems Inc following the method reported by Saldivar [Saldivar L et al., Insect Mol Biol 2008, 17(6):597-606]. 10 samples: 2 larva, 2 pre-attachment larva in B. indicus and 2 in B. taurus, and 2 adult ticks in B. indicus and 2 in B. taurus
 
Contributor(s) Rodriguez Valle M, Gondor C
Citation(s) 20637126
Submission date Mar 03, 2010
Last update date Mar 22, 2012
Contact name Manuel Rodriguez Valle
E-mail manuel.rodriguezvalle@deedi.qld.gov.au
Phone 61-733629467
Fax 61-733629429
URL http://www.dpi.qld.gov.au
Organization name DEEDI
Department Emerging Technologies
Street address 665 Fairfield road, Yeerongpilly
City Brisbane
State/province QLD
ZIP/Postal code 4103
Country Australia
 
Platforms (1)
GPL10125 Nimblegen Rhipicephalus microplus 385K microarray
Samples (10)
GSM517406 Larvae rep 1
GSM517407 Larvae rep 2
GSM517408 Brahman frustrate larvae rep 1
Relations
BioProject PRJNA124779

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20605_RAW.tar 55.6 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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