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| Status |
Public on Jun 09, 2022 |
| Title |
Physiological and transcriptomic effects of hexafluoropropylene oxide dimer acid in Caenorhabditis elegans during development |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Per- and polyfluoroalkyl substances (PFAS) are chemicals that are relatively resistant to degradation. While such features are desirable in a variety of consumer and industry products, such as firefighting foams and non-stick cookware coating, some PFAS, including perfluorooctanoic acid (PFOA), are toxic and bioaccumulate. Hexafluoropropylene oxide dimer acid (HFPO-DA) is an emerging PFAS developed to replace PFOA and has not been extensively studied. To evaluate the potential toxicity of HFPO-DA with a cost- and time-efficient approach, we exposed C. elegans larvae to 4×10-9–4 g/L HFPO-DA in liquid media and performed assays measuring developmental, behavioral, locomotor, and transcriptional effects at various exposure levels. After 48 hours of 1.5–4 g/L HFPO-DA exposure, acute developmental toxicity was observed as developmental delay; statistically significantly delayed (p < 0.05) progeny production was observed in worms exposed to 2–4 g/L HFPO-DA. After 48 hours of 4×10-9–0.4 g/L exposure, no significant behavioral or locomotor effect was observed relative to the 0 g/L control group. Statistically significant differential gene expression was identified with over 99% confidence via the R-package NOISeq in all fourteen 48-hour 1.25×10¬-5–4 g/L HFPO-DA exposure groups, except for 6.25×10¬-5 g/L. Among 10298 genes analyzed, 2624 differentially expressed genes (DEGs) were identified in the developmentally delayed 4 g/L group only, and 78 genes were differentially expressed in at least one of the thirteen exposure groups testing 1.25×10¬-5–2 g/L HFPO-DA exposures. Genes encoding for detoxification proteins such as cytochrome P450 enzymes and UDP glucuronosyltransferases are upregulated in 0.25–4 g/L acute exposure groups. In the lower exposure concentration groups, statistically significant gene expression changes were also observed, though these DEGs did not share any biological functions, except for six ribosomal protein-coding genes. While our transcriptional data is insufficient for conclusive mechanistic explanation, the statistically significant gene expression differences detected at 1.25×10¬-5 g/L, the lowest concentration tested for transcriptional changes, is concerning and calls for further targeted analyses that focus on low-dose HFPO-DA exposure effects.
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| Overall design |
Gene expression profiling analysis of shallow RNA-seq data for C. elegans 48 hour exposure to hexafluoropropylene oxide dimer acid (HFPO-DA); four replicates of 15 experimental conditions (14 different concentrations of HFPO-DA plus a control) were generated, with each condition containing around 1000 worms
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| Contributor(s) |
Feng Z, McLamb F, Vu JP, Gong S, Gersberg RM, Bozinovic G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 06, 2022 |
| Last update date |
Jun 09, 2022 |
| Contact name |
Goran Bozinovic |
| E-mail(s) |
goran@bozinstitute.org
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| Phone |
19196073155
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| Organization name |
Boz Life Science Research and Teaching Institute
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| Lab |
Boz
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| Street address |
3030 Bunker Hill St
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| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92109-5754 |
| Country |
USA |
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| Platforms (1) |
| GPL32326 |
NextSeq 2000 (Caenorhabditis elegans) |
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| Samples (60)
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| GSM6215081 |
Sample 4_Worm, 48h_0.5g/L, rep1 |
| GSM6215082 |
Sample 5_Worm, 48h_0.25g/L, rep1 |
| GSM6215083 |
Sample 6_Worm, 48h_0.125g/L, rep1 |
| GSM6215084 |
Sample 7_Worm, 48h_6.25×10^-2g/L, rep1 |
| GSM6215085 |
Sample 8_Worm, 48h_3.13×10^-2g/L, rep1 |
| GSM6215086 |
Sample 9_Worm, 48h_1.56×10^-2g/L, rep1 |
| GSM6215087 |
Sample 10_Worm, 48h_7.81×10^-3g/L, rep1 |
| GSM6215088 |
Sample 11_Worm, 48h_1.56×10^-3g/L, rep1 |
| GSM6215089 |
Sample 12_Worm, 48h_3.13×10^-4g/L, rep1 |
| GSM6215090 |
Sample 13_Worm, 48h_6.25×10^-5g/L, rep1 |
| GSM6215091 |
Sample 14_Worm, 48h_1.25×10^-5g/L, rep1 |
| GSM6215092 |
Sample 15_Worm, 48h_0g/L, rep1 |
| GSM6215093 |
Sample 16_Worm, 48h_4g/L, rep2 |
| GSM6215094 |
Sample 17_Worm, 48h_2g/L, rep2 |
| GSM6215095 |
Sample 18_Worm, 48h_1g/L, rep2 |
| GSM6215096 |
Sample 19_Worm, 48h_0.5g/L, rep2 |
| GSM6215097 |
Sample 20_Worm, 48h_0.25g/L, rep2 |
| GSM6215098 |
Sample 21_Worm, 48h_0.125g/L, rep2 |
| GSM6215099 |
Sample 22_Worm, 48h_6.25×10^-2g/L, rep2 |
| GSM6215100 |
Sample 23_Worm, 48h_3.13×10^-2g/L, rep2 |
| GSM6215101 |
Sample 24_Worm, 48h_1.56×10^-2g/L, rep2 |
| GSM6215102 |
Sample 25_Worm, 48h_7.81×10^-3g/L, rep2 |
| GSM6215103 |
Sample 26_Worm, 48h_1.56×10^-3g/L, rep2 |
| GSM6215104 |
Sample 27_Worm, 48h_3.13×10^-4g/L, rep2 |
| GSM6215105 |
Sample 28_Worm, 48h_6.25×10^-5g/L, rep2 |
| GSM6215106 |
Sample 29_Worm, 48h_1.25×10^-5g/L, rep2 |
| GSM6215107 |
Sample 30_Worm, 48h_0g/L, rep2 |
| GSM6215108 |
Sample 31_Worm, 48h_4g/L, rep3 |
| GSM6215109 |
Sample 32_Worm, 48h_2g/L, rep3 |
| GSM6215110 |
Sample 33_Worm, 48h_1g/L, rep3 |
| GSM6215111 |
Sample 34_Worm, 48h_0.5g/L, rep3 |
| GSM6215112 |
Sample 35_Worm, 48h_0.25g/L, rep3 |
| GSM6215113 |
Sample 36_Worm, 48h_0.125g/L, rep3 |
| GSM6215114 |
Sample 37_Worm, 48h_6.25×10^-2g/L, rep3 |
| GSM6215115 |
Sample 38_Worm, 48h_3.13×10^-2g/L, rep3 |
| GSM6215116 |
Sample 39_Worm, 48h_1.56×10^-2g/L, rep3 |
| GSM6215117 |
Sample 40_Worm, 48h_7.81×10^-3g/L, rep3 |
| GSM6215118 |
Sample 41_Worm, 48h_1.56×10^-3g/L, rep3 |
| GSM6215119 |
Sample 42_Worm, 48h_3.13×10^-4g/L, rep3 |
| GSM6215120 |
Sample 43_Worm, 48h_6.25×10^-5g/L, rep3 |
| GSM6215121 |
Sample 44_Worm, 48h_1.25×10^-5g/L, rep3 |
| GSM6215122 |
Sample 45_Worm, 48h_0g/L, rep3 |
| GSM6215123 |
Sample 46_Worm, 48h_4g/L, rep4 |
| GSM6215124 |
Sample 47_Worm, 48h_2g/L, rep4 |
| GSM6215125 |
Sample 48_Worm, 48h_1g/L, rep4 |
| GSM6215126 |
Sample 49_Worm, 48h_0.5g/L, rep4 |
| GSM6215127 |
Sample 50_Worm, 48h_0.25g/L, rep4 |
| GSM6215128 |
Sample 51_Worm, 48h_0.125g/L, rep4 |
| GSM6215129 |
Sample 52_Worm, 48h_6.25×10^-2g/L, rep4 |
| GSM6215130 |
Sample 53_Worm, 48h_3.13×10^-2g/L, rep4 |
| GSM6215131 |
Sample 54_Worm, 48h_1.56×10^-2g/L, rep4 |
| GSM6215132 |
Sample 55_Worm, 48h_7.81×10^-3g/L, rep4 |
| GSM6215133 |
Sample 56_Worm, 48h_1.56×10^-3g/L, rep4 |
| GSM6215134 |
Sample 57_Worm, 48h_3.13×10^-4g/L, rep4 |
| GSM6215135 |
Sample 58_Worm, 48h_6.25×10^-5g/L, rep4 |
| GSM6215136 |
Sample 59_Worm, 48h_1.25×10^-5g/L, rep4 |
| GSM6215137 |
Sample 60_Worm, 48h_0g/L, rep4 |
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| Relations |
| BioProject |
PRJNA846502 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205582_worm_normalized.csv.gz |
4.9 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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