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| Status |
Public on Oct 31, 2024 |
| Title |
Evaluating the presence of rare ERα-antagonism induced cells in WT tissues |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To examine the effects of ERα antagonism (giredestrant) on Esr1-wildtype and Esr1-mutant mammary glands, animals were treated with hormone pellets (E2, P4), then dosed daily with vehicle (MCT) or giredestrant (30 mg/kg, p.o.) for four days. Following dosing, individual mammary epithelial cell populations were FACS-isolated and sequenced. Rare giredestrant-induced novel cells (at least 1000 cells per sample) were collected from WT tissues and compared to mutant-induced novel cells.
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| Overall design |
To examine the effects of ERα antagonism (giredestrant) on Esr1-wildtype and Esr1-mutant mammary glands, animals were treated with hormone pellets (E2, P4), then dosed daily with giredestrant for four days. 18 samples are included, 3 biological replicates per condition.
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| Web link |
https://doi.org/10.1038/s43018-024-00898-8
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| Contributor(s) |
Liang J, Hafner M, Metcalfe C |
| Citation(s) |
39805955 |
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| Submission date |
Jun 06, 2022 |
| Last update date |
Jan 30, 2025 |
| Contact name |
Jackson Liang |
| E-mail(s) |
liangj14@gene.com
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| Organization name |
Genentech
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| Department |
Discovery Oncology
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| Lab |
Metcalfe Lab
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| Street address |
1 DNA Way
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| GSM6214997 |
D542G, ML, sample1 |
| GSM6214998 |
D542G, ML, sample2 |
| GSM6214999 |
D542G, ML, sample3 |
| GSM6215000 |
D542G, N, sample1 |
| GSM6215001 |
D542G, N, sample2 |
| GSM6215002 |
D542G, N, sample3 |
| GSM6215003 |
Y541S, ML, sample1 |
| GSM6215004 |
Y541S, ML, sample2 |
| GSM6215005 |
Y541S, ML, sample3 |
| GSM6215006 |
Y541S, N, sample1 |
| GSM6215007 |
Y541S, N, sample2 |
| GSM6215008 |
Y541S, N, sample3 |
| GSM6215009 |
WT, N, sample1 |
| GSM6215010 |
WT, N, sample2 |
| GSM6215011 |
WT, N, sample3 |
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| Relations |
| BioProject |
PRJNA846353 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205577_RAW.tar |
9.1 Mb |
(http)(custom) |
TAR (of TAB) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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