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Series GSE204816 Query DataSets for GSE204816
Status Public on Nov 10, 2022
Title Synergistic activation of RARb and RARg-driven programs restores cell-types specialization during stem cell differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary How cells respond to different external cues to develop along defined cell lineages composing complex tissues is a major question in systems biology. In this study, we studied the role of synthetic agonists targeting specific Retinoic acid receptors (RARs) on activating major gene regulatory wires driving cell specialization during nervous tissue formation issued from P19 stem cells. Specifically, we have revealed that the synergistic activation of the RAR and RAR nuclear receptors by their cognate ligands (BMS641 or BMS961) induce neuronal maturation, inlcuding specialized neuronal subtypes as well as to astrocytes and oligodendrocyte precursors.
With the help of Rar-null mutant lines, combined with the use of RAR-specific agonists, global transcriptome mapping and an in-silico modeling of transcription regulatory signalling propagation, we have highlighted major gene programs essential for optimal neuronal cell specialization. Overall, this study provides a systems biology view of the gene programs behind the previously observed redundancy between RAR receptors, paving the way for their potential use for directing cell specialization during nervous tissue formation.
Overall design Comparative gene expression profiling analysis of RNA-seq data for WT P19 or rar-null mutant EC cells treated with ATRA, BMS753, BMS641, BMS961 or a combination of BMS641 & BMS961 .
Contributor(s) Koshy A, Mathieux E, Stüder F, Bramoulle A, Lieb M, Colombo BM, Gronemeyer H, Mendoza-Parra MA
Citation(s) 36446525
Submission date May 25, 2022
Last update date Dec 15, 2022
Contact name Marco Antonio Mendoza Parra
Organization name Genoscope
Street address 2 Gaston Crémieux
City Evry
ZIP/Postal code 91000
Country France
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM6195538 P19, WT, ATRA, Day 2
GSM6195539 P19, WT, ATRA, Day 4
GSM6195540 P19, WT, ATRA, Day 10
BioProject PRJNA842391

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Supplementary file Size Download File type/resource
GSE204816_RAW.tar 140.0 Kb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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