|
| Status |
Public on Oct 17, 2023 |
| Title |
scGEX/scTCR-Seq of CD44+ cytotoxic T cells in LCMV-infected Wildtype and Il1rl1-ExAB-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
To study the effect of IL-33 sensing on CD8 T cell differentiation during acute infection with lymphocytic choriomeningitis virus (LCMV), activated CD44+ CTLs were flowcytometrically sorted from infected C57BL/6 wildtype and Il1rl1 gene-targeted mice (Il1rl1-ExAB-/-) and subjected to combined single-cell gene expression and single cell TCR-Seq analysis.
|
| |
|
| Overall design |
Comparative gene expression profiling analysis of the effects of IL-33 on CTLs, Th1 and Th2 cells using RNA-Seq and single-cell RNA-Seq of WT- and Il1rl1-ExAB-KO CTLs after an acute LCMV infection.
|
| |
|
| Contributor(s) |
Brunner TM, Serve SG, Marx A, Dzamukova M, Duran N, Saikali P, Kommer C, Heinrich F, Durek P, Heinz GA, Höfer T, Mashreghi M, Pinschewer DD, Kühn R, Löhning M |
| Citation(s) |
38172258 |
| |
| Submission date |
May 24, 2022 |
| Last update date |
Feb 14, 2024 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
|
| Organization name |
Deutsches Rheuma-Forschungszentrum
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL30172 |
NextSeq 2000 (Mus musculus) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE204695 |
A type 1 immunity-restricted promoter of the IL-33 receptor gene drives antiviral T cell responses |
|
| Relations |
| BioProject |
PRJNA841968 |
Less...
More...