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| Status |
Public on Dec 31, 2013 |
| Title |
PPARd target genes in MEFs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The PPAR (Peroxisome proliferator-activated receptor) family of nuclear receptors has three members: PPARg, PPARa and PPARd. Although they share similar structures, their biological functions are distinct. PPARg controls lipid storage and adipogenesis, while PPARd is associated with fat burning. The highly specific synthetic ligand for PPARd, GW501516, is a promising drug candidate for obesity and diabetes. Here we use Affymetrix microarray to analyze gene expression profile in mouse embryo fibroblasts treated with 100 nM GW501516 for 0, 2, 8 and 24 hours. These data may provide new clues into the molecular mechanism by which GW501516 ameliorates obesity and diabetes.
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| Overall design |
Wild type mouse embryonic fibroblasts (MEFs) stably infected with retroviruses MSCVpuro expressing PPARd were plated at 0.5 million per 10 cm dish. After overnight incubation, cells were treated with 100nM GW501516 for 0h, 2h, 8h and 48h. Cells were collected at subconfluent condition. Total RNAs were sequentially purified with Trizol (Invitrogen) and RNeasy kit (Qiagen) and analyzed in triplicate on Mouse Genome 430 2.0 Array (Affymetrix) at NIDDK Microarray Core Facility following standard protocols.
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| Contributor(s) |
Hong S, Ge K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 17, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Qihuang Jin |
| E-mail(s) |
jinq@niddk.nih.gov
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| Phone |
301-451-7068
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| Organization name |
NIH
|
| Department |
NIDDK
|
| Lab |
Kai Ge
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| Street address |
9000 Rockville Pike
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM510273 |
cell without GW501516 treatment replicate 1 |
| GSM510274 |
cell without GW501516 treatment replicate 2 |
| GSM510275 |
cell without GW501516 treatment replicate 3 |
| GSM510276 |
cell with GW501516 treatment for 2h replicate 1 |
| GSM510277 |
cell with GW501516 treatment for 2h replicate 2 |
| GSM510278 |
cell with GW501516 treatment for 2h replicate 3 |
| GSM510279 |
cell with GW501516 treatment for 8h replicate 1 |
| GSM510280 |
cell with GW501516 treatment for 8h replicate 2 |
| GSM510281 |
cell with GW501516 treatment for 8h replicate 3 |
| GSM510282 |
cell with GW501516 treatment for 48h replicate 1 |
| GSM510283 |
cell with GW501516 treatment for 48h replicate 2 |
| GSM510284 |
cell with GW501516 treatment for 48h replicate 3 |
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| Relations |
| BioProject |
PRJNA125473 |
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