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Series GSE2037 Query DataSets for GSE2037
Status Public on Mar 11, 2005
Title carbon utilization in E. coli
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary The study investigated the effect of different carbon sources on E. coli global gene expression. We grew MG1655 cells aerobically in MOPS minimal medium with either glucose, glycerol, succinate, L-alanine, acetate, or L-proline as the carbon supply. Samples were taken from each culture at mid log phase and were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChipĂ’ E. coli Antisense Genome Arrays as recommended in the technical manual (www.affymetrix.com).
Keywords: parallel sample
 
 
Contributor(s) Liu M, Durfee T, Cabrera J, Zhao K, Jin D, Blattner F
Citation(s) 15705577
Submission date Dec 03, 2004
Last update date Mar 16, 2012
Contact name Mingzhu Liu
E-mail(s) mingzhuliu@wisc.edu
Phone (608)-262-2534
Organization name University of Wisconsin-Madison
Street address
City Madison
State/province WI
ZIP/Postal code 53705
Country USA
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (15)
GSM37063 glucose1
GSM37064 glucose2
GSM37065 glucose3
Relations
BioProject PRJNA91865

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