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Series GSE203524 Query DataSets for GSE203524
Status Public on Jun 27, 2022
Title Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo [RNAseq mESC reciprocal cross]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Parents transmit genetic and epigenetic information to their offspring. Maternal effect genes regulate the offspring epigenome to ensure normal development. Here we report that the epigenetic regulator SMCHD1 has a maternal effect on Hox gene expression and skeletal patterning. Maternal SMCHD1, present in the oocyte and preimplantation embryo, prevents precocious activation of Hox genes post-implantation. Without maternal SMCHD1, highly penetrant posterior homeotic transformations occur in the embryo. Hox genes are decorated with Polycomb marks H2AK119ub and H3K27me3 from the oocyte throughout early embryonic development; however, loss of maternal SMCHD1 does not deplete these marks. Therefore, we propose maternal SMCHD1 acts downstream of Polycomb marks to establish a chromatin state necessary for persistent epigenetic silencing and appropriate Hox gene expression later in the developing embryo. This is a striking role for maternal SMCHD1 in long-lived epigenetic effects impacting offspring phenotype.
Our overall design it to compare between Smchd1 control and Smchd1 heterozygous samples. We have performed RNA-seq in a differentiation timecourse in 3 replicate mESC of each genotype with 4 timepoints, comparing the Smchd1 heterozygous to the control, at the matched timepoint.
 
Overall design Natalia,Benetti
 
Contributor(s) Blewitt ME
Citation(s) 35879318
Submission date May 21, 2022
Last update date Aug 06, 2022
Contact name Natalia Benetti
E-mail(s) nataliabenetti17@gmail.com
Organization name WEHI
Street address 1G Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (24)
GSM6175557 RNAseq reciprocal cross wt mESC DME LIF line 1
GSM6175558 RNAseq reciprocal cross wt mESC DME LIF line 2
GSM6175559 RNAseq reciprocal cross wt mESC DME LIF line 3
This SubSeries is part of SuperSeries:
GSE183740 Maternal Smchd1 regulates Hox gene expression and patterning in the mouse embryo
Relations
BioProject PRJNA841219

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Supplementary file Size Download File type/resource
GSE203524_benetti_ESC_RNAseq_reciprocal_cross_log2RPKM_Hox.txt.gz 7.0 Kb (ftp)(http) TXT
GSE203524_benetti_ESC_RNAseq_reciprocal_cross_log2RPKM_diff_factors.txt.gz 6.3 Kb (ftp)(http) TXT
GSE203524_benetti_ESC_RNAseq_reciprocal_cross_raw_counts_all_genes.txt.gz 2.6 Mb (ftp)(http) TXT
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