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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 05, 2022 |
| Title |
Extrathymic expression of Aire controls the induction of effective TH17 cell-mediated immune response to Candida albicans |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the effect of Candida albicans on ILC3 cells and dendritic cells these subytpes were isolated by flow cytometry, in similar fashion candida specific T-cells were obtained and subjected to bulk mRNA sequencing
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| Overall design |
mRNA profiling of conventional ILC3, MHCII+ ILC3, Aire+ ILC3 and DCs in homeostasis or stimulated by heat-killed Candida albicans. mRNA profilling of T-cells stimulated by Candida albicans, mRNA profiling of Aire-suficient or deficient Aire-expressing ILC3 cells stimulated by Candida albicans
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| Contributor(s) |
Abramson J |
| Citation(s) |
35761088 |
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| Submission date |
May 17, 2022 |
| Last update date |
Jul 05, 2022 |
| Contact name |
Jakub Abramson |
| E-mail(s) |
jakub.abramson@weizmann.ac.il
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| Organization name |
Weizmann Institute of Science
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| Department |
Department of Immunology
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| Street address |
234 Herzl st.
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| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (50)
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| GSM6160762 |
Aire-GFP+ILC3, nonstimulated, biol rep1 |
| GSM6160763 |
Aire-GFP+ILC3, nonstimulated, biol rep2 |
| GSM6160764 |
Aire-GFP+ILC3, nonstimulated, biol rep3 |
| GSM6160765 |
Aire-GFP+ILC3, stimulated, biol rep1 |
| GSM6160766 |
Aire-GFP+ILC3, stimulated, biol rep2 |
| GSM6160767 |
Aire-GFP+ILC3, stimulated, biol rep3 |
| GSM6160768 |
DC, nonstimulated, biol rep1 |
| GSM6160769 |
DC, nonstimulated, biol rep2 |
| GSM6160770 |
DC, nonstimulated, biol rep3 |
| GSM6160771 |
DC, stimulated, biol rep1 |
| GSM6160772 |
DC, stimulated, biol rep2 |
| GSM6160773 |
DC, stimulated, biol rep3 |
| GSM6160774 |
ILC3, nonstimulated, biol rep1 |
| GSM6160775 |
ILC3, nonstimulated, biol rep2 |
| GSM6160776 |
ILC3, nonstimulated, biol rep3 |
| GSM6160777 |
ILC3, stimulated, biol rep1 |
| GSM6160778 |
ILC3, stimulated, biol rep2 |
| GSM6160779 |
ILC3, stimulated, biol rep3 |
| GSM6160780 |
MHCII+ILC3, nonstimulated, biol rep1 |
| GSM6160781 |
MHCII+ILC3, nonstimulated, biol rep2 |
| GSM6160782 |
MHCII+ILC3, nonstimulated, biol rep3 |
| GSM6160783 |
MHCII+ILC3, stimulated, biol rep1 |
| GSM6160784 |
MHCII+ILC3, stimulated, biol rep2 |
| GSM6160785 |
MHCII+ILC3, stimulated, biol rep3 |
| GSM6160786 |
OTII T cells nonproliferating, WT stimulated, biol rep1 |
| GSM6160787 |
OTII T cells nonproliferating, WT stimulated, biol rep2 |
| GSM6160788 |
OTII T cells nonproliferating, WT stimulated, biol rep3 |
| GSM6160789 |
OTII T cells proliferating, WT stimulated, biol rep1 |
| GSM6160790 |
OTII T cells proliferating, WT stimulated, biol rep2 |
| GSM6160791 |
OTII T cells proliferating, WT stimulated, biol rep3 |
| GSM6160792 |
Rorc-GFP+ OTII T cells, WT stimulated, biol rep1 |
| GSM6160793 |
Rorc-GFP+ OTII T cells, WT stimulated, biol rep2 |
| GSM6160794 |
Rorc-GFP+ OTII T cells, WT stimulated, biol rep3 |
| GSM6160795 |
OTII T cells nonproliferating, KO stimulated, biol rep1 |
| GSM6160796 |
OTII T cells nonproliferating, KO stimulated, biol rep2 |
| GSM6160797 |
OTII T cells nonproliferating, KO stimulated, biol rep3 |
| GSM6160798 |
OTII T cells proliferating, KO stimulated, biol rep1 |
| GSM6160799 |
OTII T cells proliferating, KO stimulated, biol rep2 |
| GSM6160800 |
OTII T cells proliferating, KO stimulated, biol rep3 |
| GSM6160801 |
OTII T cells proliferating, KO stimulated, biol rep4 |
| GSM6160802 |
Rorc-GFP+ OTII T cells, KO stimulated, biol rep1 |
| GSM6160803 |
Rorc-GFP+ OTII T cells, KO stimulated, biol rep2 |
| GSM6160804 |
Rorc-GFP+ OTII T cells, KO stimulated, biol rep3 |
| GSM6160805 |
Rorc-GFP+ OTII T cells, KO stimulated, biol rep4 |
| GSM6160806 |
Aire-GFP+ILC3, stimulated Aire WT, biol rep1 |
| GSM6160807 |
Aire-GFP+ILC3, stimulated Aire WT, biol rep2 |
| GSM6160808 |
Aire-GFP+ILC3, stimulated Aire WT, biol rep3 |
| GSM6160809 |
Aire-GFP+ILC3, stimulated Aire KO, biol rep1 |
| GSM6160810 |
Aire-GFP+ILC3, stimulated Aire KO, biol rep2 |
| GSM6160811 |
Aire-GFP+ILC3, stimulated Aire KO, biol rep3 |
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| Relations |
| BioProject |
PRJNA838870 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE203158_DESeq2_HKCA_KO_WT_umi_counts_20210815_131414.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE203158_DESeq2_ILC3_20220512_105008.txt.gz |
15.5 Mb |
(ftp)(http) |
TXT |
| GSE203158_Deseq_all_results_20190711_115256.txt.gz |
7.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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