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| Status |
Public on Jul 19, 2022 |
| Title |
ChIP-seq to reveal the genomic binding landscape of Smad3 in renal tubular cells TCMK1 |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Smad3 is an important downstream transcriptional factor of TGF-β signaling in the pathogenesis of renal fibrosis. Understanding the target genes is essential to decode the mechanism underlying Smad3-regulated renal fibrosis. To uncover the potential target genes of Smad3 in renal tubular cells, we applied the ChIP-seq technology to analyze the genomic chromatin binding landscape of Smad3 in TCMK-1 cells. The TCMK-1 cells of 90% confluence were starved in low-serum (0.5% FBS) medium for 6 h followed by stimulation with 5 ng/ml TGF-β1 for 30 min. The cells were harvested for ChIP assay. The precipitated chromatin was used for DNA isolation and deep-sequencing.
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| Overall design |
ChIP-seq for Smad3 in TGF-β1-stimulated renal tubular cell TCMK-1.
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| Contributor(s) |
Wang H, Wang L, Peng Z, Li Y |
| Citation(s) |
36091385 |
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| Submission date |
May 15, 2022 |
| Last update date |
Oct 20, 2022 |
| Contact name |
Honglian Wang |
| E-mail(s) |
honglianwang@swmu.edu.cn
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| Phone |
+8615108472354
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| Organization name |
the Affiliated Traditional Medicine Hospital of Southwest Medical University
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| Department |
Research Center for Integrative Medicine
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| Lab |
Research Center for Integrative Medicine
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| Street address |
No. 182, Chunhui Road, Longmatan District
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| City |
Luzhou |
| State/province |
Sichuan |
| ZIP/Postal code |
646000 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA838379 |
