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| Status |
Public on May 16, 2022 |
| Title |
scRNA-seq to study features of CD4conv and Treg cells in spleen and bone marrow of mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of the study was to compare gene experession between Treg cells in the bone marrow and the spleen
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| Overall design |
2 male Foxp3-DTR-GFP were used for the study. Single cells suspension from BM and spleen were prepared, then enriched for CD4 by magneic beads and sorted by gating on CD4+TCRb+. GFP was used to differentiate between Tregs and CD4conv. CD4conv or Tregs cells from the same tissue were pooled from the two mice and hashtagged (total of 4 groups: Tregs BM/Tregs spleen/CD4 conv BM/CD4 conv spleen). The mice were not treated by DT.
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| Contributor(s) |
Elias S, Schizas M, Rudensky AY |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 12, 2022 |
| Last update date |
May 18, 2022 |
| Contact name |
Michail Schizas |
| E-mail(s) |
schizasm@mskcc.org
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| Organization name |
Memorial sloan Kettering
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| Street address |
1275 York ave
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| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (1) |
| GSM6135105 |
sc-RNAseq of CD4conv and Treg cells in BM and spleen |
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| Relations |
| BioProject |
PRJNA837594 |
