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| Status |
Public on Oct 04, 2022 |
| Title |
Murine models of pancreatic cancer: KPC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We harvested and sequenced the spontaneous pancreatic tumor generated by a 6-month-old KPC mouse (KrasLSL-G12D; Trp53LSL-R172H; Ptf1a-Cre).
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| Overall design |
This 10X Genomics single-cell RNA sequencing repository includes raw and processed data files from a pancreatic KPC tumor. Single-cell cDNA libraries were prepared and sequenced at the University of Michigan Advanced Genomics Core using the 10x Genomics platform. The sample was run using paired-end 50 cycle reads on NovaSeq 6000 (Illumina) to a depth of 100,000 reads. Alignment was performed by the University of Michigan Advanced Genomics Core. Parameters include Cell Ranger version 3.1.0 (default settings), initial expected cell count of 10,000.
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| Contributor(s) |
Pasca di Magliano M, Donahue K, Steele N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 10, 2022 |
| Last update date |
Oct 06, 2022 |
| Contact name |
Marina Pasca di Magliano |
| E-mail(s) |
marinapa@umich.edu
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| Organization name |
University of Michigan Medical School
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| Street address |
1500 E Medical Center Dr
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48103 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA836890 |
