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| Status |
Public on Sep 27, 2023 |
| Title |
Glial progenitor heterogeneity, key regulators and cell signaling revealed by single-cell RNA-sequencing provide insights to regeneration in spinal cord injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Recent studies in brain and spinal cord have revealed the heterogenous nature of astrocytes; however, how diverse constituents of astrocyte lineage cells are regulated in adult spinal cord after injury and contribute to regeneration remains elusive. We performed single-cell RNA-sequencing (scRNA-seq) of astrocyte lineage cells from sub-chronic spinal cord injury (SCI) models, identified and compared with the subpopulations in the acute stage data. We found the subpopulations with distinct functional enrichment and their identities defined by subpopulation-specific transcription factors and regulons. Our analyses revealed the molecular signature, location and morphologies of potential residential neural progenitors or neural stem cells in the adult spinal cord before and after injury, and the intermediate cells enriched in neuronal markers that could potentially transition into other subpopulations. The investigation of stage-specific cell-cell communications among astrocyte lineage cells and with other cell types in the tissue generated valuable insight into signaling pathway networks in SCI. This study has significantly expanded the knowledge of the heterogeneity and cell state transition of glial progenitors in adult spinal cord before and after injury.
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| Overall design |
We performed single-cell RNA-sequencing (scRNA-seq) of astrocyte lineage cells from sub-chronic spinal cord injury (SCI) models, identified and compared with the subpopulations in the acute stage data.
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| Contributor(s) |
Wei H, Wu X, Duran RC, Chaboub L, Rolfe A, Horner PJ, Wu J |
| Citation(s) |
37149868 |
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| Submission date |
May 10, 2022 |
| Last update date |
Sep 28, 2023 |
| Contact name |
Haichao Wei |
| E-mail(s) |
haichaowei1985@gmail.com
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| Phone |
3462479690
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| Organization name |
UTHealth
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| Street address |
1825 Pressler St., SRB 637.C
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA836851 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE202627_RAW.tar |
173.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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