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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 01, 2022 |
| Title |
Effect of LUCAT1 depletion in THP-1 monocytes on gene expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the effect of LUCAT1 depletion on gene expression after stimulation with LPS or infection with Sendai virus, RNA-seq on THP-1 control guide RNA and LUCAT1-deficient cells after stimulation.
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| Overall design |
LUCAT1-deficient cells were generated using CRISPR/Cas9 system and cells transduced with a non-targeting guide RNA were used as a control. Two independent experiments per treatment and per genotype were performed.
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| Contributor(s) |
Fitzgerald KA, Vierbuchen T |
| Citation(s) |
36577072 |
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| Submission date |
May 06, 2022 |
| Last update date |
Nov 26, 2024 |
| Contact name |
Katherine A Fitzgerald |
| E-mail(s) |
kate.fitzgerald@umassmed.edu
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| Phone |
5088566518
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| Organization name |
Umass Chan Medical
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| Department |
Medicine
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| Street address |
364 Plantation Street
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| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| GSM6119063 |
THP-1, control gRNA (NTC), non-treated, biol rep 1 [NTC_NT_rep1] |
| GSM6119064 |
THP-1, control gRNA (NTC), non-treated, biol rep 2 [NTC_NT_rep2] |
| GSM6119065 |
THP-1, control gRNA (NTC), LPS 1h, biol rep 1 [NTC_LPS_1h_rep1] |
| GSM6119066 |
THP-1, control gRNA (NTC), LPS 1h, biol rep 2 [NTC_LPS1h_rep2] |
| GSM6119067 |
THP-1, control gRNA (NTC), LPS 6h, biol rep 1 [NTC_LPS6h_rep1] |
| GSM6119068 |
THP-1, control gRNA (NTC), LPS 6h, biol rep 2 [NTC_LPS6h_rep2] |
| GSM6119069 |
THP-1, control gRNA (NTC), SeV, biol rep 1 [NTC_SeV_rep1] |
| GSM6119070 |
THP-1, control gRNA (NTC), SeV, biol rep 2 [NTC_SeV_rep2] |
| GSM6119071 |
THP-1, LUCAT1-KO, non-treated, biol rep 1 [LUCAT1_NT_rep1] |
| GSM6119072 |
THP-1, LUCAT1-KO, non-treated, biol rep 2 [LUCAT1_NT_rep2] |
| GSM6119073 |
THP-1, LUCAT1-KO, LPS 1h, biol rep 1 [LUCAT1_LPS1h_rep1] |
| GSM6119074 |
THP-1, LUCAT1-KO, LPS 1h, biol rep 2 [LUCAT1_LPS1h_rep2] |
| GSM6119075 |
THP-1, LUCAT1-KO, LPS 6h, biol rep 1 [LUCAT1_LPS6h_rep1] |
| GSM6119076 |
THP-1, LUCAT1-KO, LPS 6h, biol rep 2 [LUCAT1_LPS6h_rep2] |
| GSM6119077 |
THP-1, LUCAT1-KO, SeV, biol rep 1 [LUCAT1_SeV_rep1] |
| GSM6119078 |
THP-1, LUCAT1-KO, SeV, biol rep 2 [LUCAT1_SeV_rep2] |
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| Relations |
| BioProject |
PRJNA835864 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE202409_DESeq_comparisons.csv.gz |
132.8 Kb |
(ftp)(http) |
CSV |
| GSE202409_genes_expression_raw_count.tsv.gz |
2.2 Mb |
(ftp)(http) |
TSV |
| GSE202409_genes_expression_tpm.tsv.gz |
2.2 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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