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Series GSE20176 Query DataSets for GSE20176
Status Public on Apr 02, 2010
Title Orchestration of Floral Initiation by APETALA1
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The MADS-domain transcription factor APETALA1 (AP1) is a key regulator of Arabidopsis flower development. To understand the molecular mechanisms underlying AP1 function, we identified its target genes during floral initiation using a combination of gene expression profiling and genome-wide binding studies. Many of its targets encode transcriptional regulators, including known floral repressors. The latter genes are down-regulated by AP1, suggesting that it initiates floral development by abrogating the inhibitory effects of these genes. While AP1 acts predominantly as a transcriptional repressor during the earliest stages of flower development, regulatory genes known to be required for floral organ formation were found to be activated by AP1 at more advanced stages, indicating a dynamic mode of action. Our results further imply that AP1 orchestrates floral initiation by integrating growth, patterning and hormonal pathways.
Overall design We used the AP1-GR system to conduct chromatin immunoprecipitation experiments with AP1-specific antibodies followed by deep-sequencing (ChIP-Seq) in order to determine AP1 binding sites on a genome-wide scale. Samples were generated from tissue in which the AP1-GR protein was induced for 2h using a single treatment of 1 uM DEX to the shoot apex. As control, we performed ChIP experiments using the same antibody on uninduced tissue. Experiments were done in two biological replicates.
Contributor(s) Kaufmann K, Muino JM
Citation(s) 20360106
Submission date Feb 03, 2010
Last update date Sep 04, 2019
Contact name Gerco Angenent
Organization name Plant Research International
Street address Bornsesteeg 65
City Wageningen
ZIP/Postal code 6708 PD
Country Netherlands
Platforms (1)
GPL9062 Illumina Genome Analyzer (Arabidopsis thaliana)
Samples (4)
GSM506208 AP1-GR_2h_induced_sample_1
GSM506209 AP1-GR_2h_induced_sample_2
GSM506210 AP1-GR_uninduced_control_1
SRA SRP002174
BioProject PRJNA125645

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20176_RAW.tar 128.3 Mb (http)(custom) TAR (of SOA)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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