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| Status |
Public on Apr 21, 2022 |
| Title |
ATAC-seq chromatin accessibility profiling in pancreatic islets isolated from Arid1aFl/Fl ; MIP-rtTA control and Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice (Arid1a βKO) with/without pancreatectomy |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To determine how ARID1A loss might regulate genes that were preferentially regulated on the gene expression level, we performed an assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) on islets isolated from control and Arid1a β-cell knockout (βKO) mice at baseline and 6 days after 50% pancreatectomy (PPx).
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| Overall design |
In vivo ATAC-Seq on islets isolated from 3-month-old male control and Arid1a βKO mice at baseline and 6 days after PPx (Pre-PPx: 4 Arid1aFl/Fl; MIP-rtTA control and 3 Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice, Post-PPx: 4 Arid1aFl/Fl; MIP-rtTA control and 4 Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice, all males).
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| Contributor(s) |
Celen C, Jia Y, Lu T, Zhu H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 18, 2022 |
| Last update date |
Apr 21, 2022 |
| Contact name |
Hao Zhu |
| E-mail(s) |
hao.zhu@utsouthwestern.edu
|
| Organization name |
UT Southwestern Medical Center
|
| Street address |
5323 Harry Hines Boulevard
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (15)
|
| GSM6048427 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 2] |
| GSM6048428 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 3] |
| GSM6048429 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 6] |
| GSM6048430 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 8] |
| GSM6048431 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 4] |
| GSM6048432 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 5] |
| GSM6048433 |
Pre-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 7] |
| GSM6048434 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 11] |
| GSM6048435 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 12] |
| GSM6048436 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 13] |
| GSM6048437 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA control [Sample 18] |
| GSM6048438 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 10] |
| GSM6048439 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 14] |
| GSM6048440 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 16] |
| GSM6048441 |
Post-PPx: Arid1aFl/Fl; MIP-rtTA; TRE-Cre (Arid1a βKO) [Sample 17] |
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| This SubSeries is part of SuperSeries: |
| GSE201004 |
RNA-seq and ATAC-seq in pancreatic islets isolated from mice with/without pancreatectomy |
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| Relations |
| BioProject |
PRJNA827843 |
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