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| Status |
Public on May 29, 2024 |
| Title |
Genome-wide enrichment of YAP and TEAD1 stimulated by lactate |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The goal of this study is to find out the genome-wide occupancy of YAP and TEAD1 under lactate stimulation.
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| Overall design |
293FT cells were transfected with HA-YAP and Flag-TEAD1 and cultured in glucose-free DMEM supplemented with 25mM lactate. 24h after lactate treatment, cells were collected for ChIP-seq analysis using HA and Flag antibodies.
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| Contributor(s) |
Ju J, Jiao S, An L |
| Citation(s) |
38512451 |
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| Submission date |
Apr 14, 2022 |
| Last update date |
May 29, 2024 |
| Contact name |
junyi ju |
| E-mail(s) |
jujunyi@nju.edu.cn
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| Organization name |
Shanghai Tenth People’s Hospital
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| Street address |
301 middle yanchang road
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| City |
shanghai |
| ZIP/Postal code |
200072 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| GSM6045376 |
293FT cell, control, HA-IP, rep2 |
| GSM6045377 |
293FT cell, control, Flag-IP, rep1 |
| GSM6045378 |
293FT cell, control, Flag-IP, rep2 |
| GSM6045379 |
293FT cell, lactate, input, rep1 |
| GSM6045380 |
293FT cell, lactate, input, rep2 |
| GSM6045381 |
293FT cell, lactate, HA-IP, rep1 |
| GSM6045382 |
293FT cell, lactate, HA-IP, rep2 |
| GSM6045383 |
293FT cell, lactate, Flag-IP, rep1 |
| GSM6045384 |
293FT cell, lactate, Flag-IP, rep2 |
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| Relations |
| BioProject |
PRJNA826909 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE200850_Ctrl-Flag_RPKM.bw |
313.9 Mb |
(ftp)(http) |
BW |
| GSE200850_Ctrl-Flag_vs_Lac-Flag.bed.gz |
10.5 Kb |
(ftp)(http) |
BED |
| GSE200850_Ctrl-HA_RPKM.bw |
398.3 Mb |
(ftp)(http) |
BW |
| GSE200850_Ctrl-HA_vs_Lac-HA.bed.gz |
9.4 Kb |
(ftp)(http) |
BED |
| GSE200850_Ctrl-input_RPKM.bw |
258.5 Mb |
(ftp)(http) |
BW |
| GSE200850_Lac-Flag_RPKM.bw |
400.0 Mb |
(ftp)(http) |
BW |
| GSE200850_Lac-HA_RPKM.bw |
375.0 Mb |
(ftp)(http) |
BW |
| GSE200850_Lac-input_RPKM.bw |
436.4 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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