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| Status |
Public on Jul 07, 2022 |
| Title |
Transcriptomes Analysis of 4NQO induced-esophageal tumors of Wild Type and S100A14 CKO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To fully realize the potential molecular mechanism that S100A14 deficiency enhances esophageal carcinogenesis
Methods: Total RNA of esophageal tumors in 4NQO WT and 4NQO S100A 14 CKO group mice was extracted with TRIzol Reagent. RNA libraries were constructed using an Illumina TruSeq RNA Sample Preparation kit according to the manufacturer’s protocol. A total of 150 base paired-end reads were sequenced using the Novaseq 6000 S4 platform. The read alignment was conducted using TopHat 2.0.13, and relative transcript abundances and differentially expressed genes were determined using the DESeq R package.
Results: We identified 14,157 transcripts in esophageal tumors of 4NQO WT and 4NQO S100A14 CKO group mice. Approximately 8% of the transcripts showed differential expression between the esophageal tumors of 4NQO WT and 4NQO S100A 14 CKO group mice, with a fold change ≥1.0 and p value <0.05.
Conclusions: Our study represents the first analysis of esophageal tumors derived from S100A14 CKO mice with biologic replicates, generated by RNA-seq.
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| Overall design |
mRNA profiles of 4NQO induced-esophageal tumors of Wild Type and S100A14 CKO mice
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| Contributor(s) |
Li X, Chen H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 13, 2022 |
| Last update date |
Jul 08, 2022 |
| Contact name |
Xukun Li |
| E-mail(s) |
xk_li1987@126.com
|
| Phone |
13718129906
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| Organization name |
NCC
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| Department |
Department of Radiation Oncology
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| Street address |
No.17 Panjiayuan Nanli, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100021 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA826519 |
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