|
| Status |
Public on May 10, 2024 |
| Title |
Decitabine induces the expression of genes in THP-1 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Next Generation Sequencing Facilitates Quantitative Analysis of THP-1 cells treated with DMSO and Decitabine
|
| |
|
| Overall design |
mRNA profiles of THP-1 cells were generated by deep sequencing, in duplicate, using Illumina Navoseq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays.
|
| |
|
| Contributor(s) |
Guo H, Feng R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 08, 2022 |
| Last update date |
May 10, 2024 |
| Contact name |
hezhou guo |
| E-mail(s) |
344796247@qq.com
|
| Organization name |
Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
|
| Street address |
Shanghai, P. R. China. No. 197 Rui Jin Er Road
|
| City |
shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA824849 |
Less...
More...