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Series GSE200486 Query DataSets for GSE200486
Status Public on Dec 28, 2023
Title 16S V3-V4 -based microbial profiling of stool, skin and saliva samples of patients affected by systemic sclerosis (SSc)
Organisms human skin metagenome; human saliva metagenome; human feces metagenome
Experiment type Other
Summary Systemic sclerosis (SSc) is a complex and still unclear rare disease associated with fibrosis in multiple organs. Microbiota has recently emerged as an important environmental factor in SSc pathogenesis, either at gut, oral and skin level.
 
Overall design DNA extraction from stool, skin and saliva samples collected from 26 patients affected by SSc, generatoin of amplicons of the variable V3–V4 region of the bacterial 16S rRNA gene and paired-end sequencing (2 × 300 cycles) on the Illumina MiSeq platform.
 
Citation(s) 37154625
Submission date Apr 08, 2022
Last update date Dec 28, 2023
Contact name Matteo Ramazzotti
E-mail(s) matteo.ramazzotti@unifi.it
Organization name University of Florence
Department Experimental and Clinical Biomedical Sciences
Street address viale Morgagni 50
City Firenze
ZIP/Postal code 50134
Country Italy
 
Platforms (3)
GPL29470 Illumina MiSeq (human feces metagenome)
GPL31025 Illumina MiSeq (human saliva metagenome)
GPL32156 Illumina MiSeq (human skin metagenome)
Samples (78)
GSM6034915 Feces of a patient affected by limited (ACA) sclerodermia [F_01_L]
GSM6034916 Feces of a patient affected by diffuse (anti Scl70/RNA Pol III) sclerodermia [F_02_D]
GSM6034917 Feces of a patient affected by diffuse (anti Scl70/RNA Pol III) sclerodermia [F_03_D]
Relations
BioProject PRJNA824808

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE200486_otu_tax.txt.gz 74.1 Kb (ftp)(http) TXT
GSE200486_otus.fasta.gz 202.3 Kb (ftp)(http) FASTA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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