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| Status |
Public on Nov 02, 2022 |
| Title |
Role of OspC effectors in the IFN response |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interferons (IFNs) are vital weapons in the arsenal of immune mediators produced in response to infection. We showed that Shigella sonnei blocks signaling and interferon-stimulated genes (ISGs) downstream of all families of IFN via secreted effectors of the OspC family. Notably, Ospc1 and OspC3 strongly block the IFN response. This project aims at investigating the cellular signaling pathways that are impacted by OspC1 and OspC3 with or without IFN stimulation and at clarifying whether observed changes in ISGs expression are general or specific to some ISGs.
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| Overall design |
293T cells expressing either empty GFP, OspC1 or OspC3 were treated or not with IFN beta overnight. Samples are provided as biological triplicates (experiments performed on different days), resulting in 3 vectors x 2 IFN treatments x triplicates =18 samples.
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| Contributor(s) |
Alphonse N, Odendall C, Wack A |
| Citation(s) |
35568036 |
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| Submission date |
Apr 07, 2022 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Andreas Wack |
| E-mail(s) |
andreas.wack@crick.ac.uk
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| Organization name |
The Francis Crick Institute
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| Lab |
Immunoregulation Laboratory
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| Street address |
1 Midland Road
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| City |
London |
| ZIP/Postal code |
NW1 1AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (18)
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| GSM6033834 |
Overexpressing GFP, beta-treated [ALP4689A22] |
| GSM6033835 |
Overexpressing OspC1, beta-treated [ALP4689A23] |
| GSM6033836 |
Overexpressing OspC3, beta-treated [ALP4689A24] |
| GSM6033837 |
Overexpressing GFP, UT [ALP4689A25] |
| GSM6033838 |
Overexpressing OspC1, UT [ALP4689A26] |
| GSM6033839 |
Overexpressing OspC3, UT [ALP4689A27] |
| GSM6033840 |
Overexpressing GFP, beta-treated [ALP4689A28] |
| GSM6033841 |
Overexpressing OspC1, beta-treated [ALP4689A29] |
| GSM6033842 |
Overexpressing OspC3, beta-treated [ALP4689A30] |
| GSM6033843 |
Overexpressing GFP, UT [ALP4689A31] |
| GSM6033844 |
Overexpressing OspC1, UT [ALP4689A32] |
| GSM6033845 |
Overexpressing OspC3, UT [ALP4689A33] |
| GSM6033846 |
Overexpressing GFP, beta-treated [ALP4689A34] |
| GSM6033847 |
Overexpressing OspC1, beta-treated [ALP4689A35] |
| GSM6033848 |
Overexpressing OspC3, beta-treated [ALP4689A36] |
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| Relations |
| BioProject |
PRJNA824517 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE200447_Normalizedcounts.txt.gz |
2.9 Mb |
(ftp)(http) |
TXT |
| GSE200447_README.txt |
583 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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