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Series GSE20039 Query DataSets for GSE20039
Status Public on Jan 27, 2010
Title Expression data from Arabidopsis seed compartments at the bending coyledon stage
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We collected bending-cotyledon seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing bending cotyledon stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, mycropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, in addition to serial sections encompasing the entire bending-cotyledon stage seed.
 
Overall design Bending-cotyledon stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).
 
Contributor(s) Kirkbride RC, Belmonte MF, Fei J, Hashimoto MM, Stone SL, Pelletier J, Cheng C, Golberg RB, Harada JJ
Citation(s) 23319655, 25684030
Submission date Jan 26, 2010
Last update date Aug 15, 2018
Contact name John J Harada
E-mail(s) jjharada@ucdavis.edu
Phone 530-752-0673
Organization name University of California, Davis
Department Plant Biology
Lab Harada
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (14)
GSM501157 Arabidopsis bEP1
GSM501158 Arabidopsis bEP2
GSM501159 Arabidopsis bPEN1
This SubSeries is part of SuperSeries:
GSE12404 Expression data from Arabidopsis Seed Compartments at 5 discrete stages of development
Relations
BioProject PRJNA124299

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20039_RAW.tar 26.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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