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| Status |
Public on Feb 01, 2010 |
| Title |
Minicircle-derived iPS cells - Affymetrix Data |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Third-party reanalysis
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| Summary |
Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem (iPS) cells. We report the use of ‘minicircle’ DNA, a vector type that is free of bacterial DNA and capable of high expression in cells. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. (Note: Our Nature Methods publication included analysis of array data from GSM378832 (Foreskin), GSM378833-GSM378838 (JT-iPSC), and GSM378817-GSM378820 (H1, H7, H9, H13, H14) in conjunction with this series).
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| Overall design |
Total RNA from human adipose stem cells (hASC, n = 3 replicate samples), hASC-derived iPS cells using lentiviral factors (lenti-iPSC, n = 3 replicate samples), and minicircle-derived human iPS cells (mc-iPSC, n = 3 subclones from adipose tissue of three individual patients) was hybridized to nine Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays.
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| Contributor(s) |
Wilson KD, Jia F, Sun N |
| Citation(s) |
20139967 |
| Submission date |
Jan 25, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Kitchener D. Wilson |
| E-mail(s) |
kitchwilson@stanford.edu
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| Organization name |
Stanford University
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| Street address |
S140 Grant Bldg
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA124121 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20033_RAW.tar |
93.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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