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| Status |
Public on Jun 24, 2024 |
| Title |
CK2 phosphorylation of cap methyltransferase CMTR1 promotes RNAPII interaction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated influence of CMTR1 phosphorylation by CK2 on RNA expression. In this experiment, we added wild-type and phosphorylation deficient mutant of CMTR1 and knocked out the endogenous CMTR1. We demonstrated that lack of phosphorylation of CMTR1 affects only small but specific group of genes.
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| Overall design |
RNA sequencing of total RNA from mouse embroynic fibroblasts transduced with different variants of HA-tagged CMTR1 and treated with Cre recombinase to remove CMTR1 exon 3 flanked by loxP sites
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| Contributor(s) |
Lukoszek R, Cowling VH |
| Citation(s) |
38923463 |
| |
| Submission date |
Apr 04, 2022 |
| Last update date |
Sep 23, 2024 |
| Contact name |
Radoslaw Lukoszek |
| E-mail(s) |
rlukoszek@gmail.com
|
| Organization name |
University of Potsdam
|
| Street address |
Karl-Liebknecht-Strasse 24-25
|
| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA823002 |
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