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| Status |
Public on Apr 04, 2022 |
| Title |
Sequencing and Quantitative Analysis of Skin Transcriptomes from Rats with EGFR Inhibitors Induced Rash |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Skin mRNA profiles of wild-type (WT) and EGFR Inhibitors (Gefitinib and Afatinib) induced rash rats were generated by deep sequencing, in triplicate, using Illumina GAIIx. Raw sequences were mapped to the rat reference sequence by Hisat2 v2.0.5. FeatureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene. Differential expression analysis was performed using the DESeq2 R package (1.16.1).
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| Overall design |
Skin mRNA profiles of wild-type (WT) and EGFR Inhibitors (Gefitinib and Afatinib) induced rash rats
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| Contributor(s) |
Zhang S, Liu X, Xie X, Chen L |
| Citation(s) |
35618045 |
| |
| Submission date |
Apr 01, 2022 |
| Last update date |
Jul 05, 2022 |
| Contact name |
Shiyi Zhang |
| E-mail(s) |
zhangshiyi@sjtu.edu.cn
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| Organization name |
Shanghai Jiao Tong University
|
| Street address |
800 Dongchuan Rd
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| City |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA822384 |
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