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| Status |
Public on Sep 29, 2023 |
| Title |
Using Zinc-based probes to generate pseudoislets from purified human alpha-cells for functional analyses |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Investigation of the function of human alpha-cells remains a challenge due to the lack of simple and cost-effective purification methods to isolate high-quality alpha-cells from islets. Here, we introduce a novel and simple method for enriching live alpha-cells from dissociated human islet cells by using the reaction-based probe diacetylated Zinpyr1 (DA-ZP1) with > 97% purity.
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| Overall design |
Sorted islet cells from four islet donors were seeded in U-bottom low attachment 96 well plates (1000 cells/200 ul/well). Half of the medium was refreshed every other day for 10 days.
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| Contributor(s) |
Kahraman S, Kulkarni RN |
| Citation(s) |
37732504 |
| |
| Submission date |
Mar 25, 2022 |
| Last update date |
Oct 24, 2023 |
| Contact name |
Jonathan M Dreyfuss |
| Organization name |
Joslin Diabetes Center
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| Department |
Bioinformatics & Biostatistics Core
|
| Street address |
1 Joslin Pl
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (33)
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| Relations |
| BioProject |
PRJNA819965 |
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