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| Status |
Public on Dec 28, 2022 |
| Title |
RNA-seq and ChIP-seq in HepG2 hepatocellular carcinoma and U87 glioblastoma cells with CRISPRi knockdown of ACTR5 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This dataset examined the effect of ACTR5 knockdown in HepG2 and U87 cells. The gene expression profiling (RNA-seq) and the chromatin targeting of ACTR5 (TST-ChIP-seq) are reported.
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| Overall design |
RNAseq:dCas9-Krab-expressing HepG2 and U87 cells were transduced with sgiNTC and sgiACTR5. The total RNA was extracted using the RNeasy Mini Kit (74104, QIAGEN) and submitted for RNA-seq at Novogene (PE150, NovoSeq6000). TST-ChIP-seq: ACTR5-TST cautured genomic DNA was submitted for ChIP-seq at Novogene (PE150, NovaSeq6000).
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| Contributor(s) |
Chen C, Yang L, Xu X |
| Citation(s) |
36563143 |
| |
| Submission date |
Mar 22, 2022 |
| Last update date |
Dec 29, 2022 |
| Contact name |
LU YANG |
| E-mail(s) |
luyang@coh.org
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| Organization name |
city of hope
|
| Street address |
1500 duarte rd
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| City |
duarte |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA818644 |
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