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| Status |
Public on Oct 18, 2022 |
| Title |
Generation of human kidney organoids from urine cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Kidney organoids are ideal models to study the complex process of human kidney development. Here we report the generation of functional kidney organoids by reprogramming human urine epithelial cells (hUCs). RNA-seq and ATAC-seq revealed the three-stage process of the 2D U-iRO induction. Single cell RNA-seq further reveals the cell types in 2D and 3D organoids, 2D U-iRO dominated with mesenchyme and 3D U-iRO with tubule.
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| Overall design |
We analyzed the transcriptome expression profiles (RNA-seq) and chromation accessibility (ATAC-seq) during 2D U-iRO induction. 10X Chromium were used to profile 2D and 3D U-iRO
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| Citation(s) |
36243732 |
| |
| Submission date |
Mar 17, 2022 |
| Last update date |
Oct 31, 2022 |
| Contact name |
huan chen |
| E-mail(s) |
chen_huan@grmh-gdl.cn
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| Organization name |
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)
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| Street address |
Huangpu District
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| City |
guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| This SuperSeries is composed of the following SubSeries: |
| GSE198838 |
Generation of human kidney organoids from urine cells (ATAC-seq) |
| GSE198839 |
Generation of human kidney organoids from urine cells (RNA-seq) |
| GSE198840 |
Generation of human kidney organoids from urine cells (scRNA-Seq) |
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| Relations |
| BioProject |
PRJNA817161 |
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