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| Status |
Public on Aug 31, 2022 |
| Title |
Transcriptional profile of NOMO1 knockout cell lines [Affymetrix] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We report the data of whole Gene expression of three different cell lines, HT-29 (early-onset colorectal cancer, EOCRC), HCT-116 (EOCRC) and HS-5 (bone marrow) in order to analyze the transcriptional profile generated after NOMO1 (Nodal Modulator 1) gene inactivation, a candidate biomarker in EOCRC. The generation of the NOMO1 knockout cell lines was performed using CRISPR/cas9 technology. The main objective was to identify whether the loss of NOMO1 generated a differential transcriptional profile in each cell line and whether it was possible to identify genes commonly deregulated in the three NOMO1-KO cell lines. The analysis revealed a set of differentially expressed genes in common to the three cell lines as a consequence of NOMO1 inactivation.
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| Overall design |
Examination of the transcriptional profile of HT-29, HCT-116 and HS-5 NOMO1 knockout cell lines.
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| Contributor(s) |
Pérez García J, Martel-Martel A, García-Vallés P, Corchete L, García JL, Gestoso-Uzal N, Vidal Tocino R, Blanco O, Méndez L, Sánchez-Martín M, Fuentes M, Herrero AB, Holowatyj AN, Perea J, González-Sarmiento R |
| Citation(s) |
36011023 |
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| Submission date |
Mar 10, 2022 |
| Last update date |
Sep 01, 2022 |
| Contact name |
Rogelio González-Sarmiento |
| E-mail(s) |
gonzalez@usal.es
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| Organization name |
University of Salamanca
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| Department |
Medicine
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| Lab |
Molecular Medicine
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| Street address |
Alfonso X el Sabio, sn
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| City |
Salamanca |
| State/province |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
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| Platforms (1) |
| GPL32050 |
[Clariom_S_Human] Affymetrix Clariom S Human array [ClariomSHuman_Hs_ENSG_25.0.0] |
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| Samples (14)
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| GSM5945067 |
HT-29, NOMO KO-2 |
| GSM5945068 |
HCT-116, WT |
| GSM5945069 |
HCT-116, PX458-1 |
| GSM5945070 |
HCT-116, NOMO KO-1 |
| GSM5945071 |
HCT-116, NOMO KO-2 |
| GSM5945072 |
HS-5, PX458-1 |
| GSM5945073 |
HS-5, PX458-2 |
| GSM5945074 |
HS-5, PX458-3 |
| GSM5945075 |
HS-5, NOMO KO-1 |
| GSM5945076 |
HS-5, NOMO KO-2 |
| GSM5945077 |
HS-5, NOMO KO-3 |
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| This SubSeries is part of SuperSeries: |
| GSE198383 |
Transcriptional profile of NOMO1 knockout cell lines |
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| Relations |
| BioProject |
PRJNA814634 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198324_RAW.tar |
20.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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