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| Status |
Public on Sep 13, 2023 |
| Title |
Genome-wide maps of hematopoietic stem and progenitor cells in mice bone marrow. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To investigate whether the skeletal retardation observed in NG2-cre/Pot1afl/fl mice affected hematopoiesis.
Methods: Whole bone marrow cells mRNA profiles of 2-week-old wild-type (NG2-cre/Pot1awt/wt) and Pot1a knockout (NG2-cre/Pot1afl/fl) mice were generated by deep sequencing, using Illumina NextSeq 500.
Results: We compared the relative sizes of these subpopulations between the NG2-cre/Pot1afl/fl and control mice and found a significant reduction in cells of the B-cell lineage in NG2-cre/Pot1afl/fl marrow. Cell subpopulations associated with B-cell differentiation from hematopoietic stem/progenitor cells (HSPCs) were identified according to the expression of established marker genes.
Conclusions: Bone marrow microenvironments composed of POT1a-deleted MSPCs impair B-cell development.
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| Overall design |
Single cell mRNA profiles of bone marrow HSCs and progenitor cells in NG2-cre/Pot1afl/fl and control mice.
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| Contributor(s) |
Nakashima K, Kunisaki Y, Semba Y, Nogami J, Arai F |
| Citation(s) |
37773433 |
| |
| Submission date |
Mar 01, 2022 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Yuichiro Semba |
| E-mail(s) |
semba.yuichiro.208@m.kyushu-u.ac.jp
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| Organization name |
Kyushu University
|
| Street address |
3-1-1 Maidashi
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA811630 |
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