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| Status |
Public on Nov 05, 2023 |
| Title |
Short C-terminal Musashi-1 proteins regulate pluripotency states in embryonic stem cells |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
To understand the function of MSI1 in pluripotent stem cells, RNA-seq assays were performed on mouse embryonic stem cells R1, MSI1 knockout cell line R1-C5, human embryonic stem cells H9, RRM knockout cell line H9-C8, MSI1 full-length overexpression cell line H9-MSI1OE, MSI1C variant overexpression cell line H9-MSI1 (138-362) OE , H9-MSI1(272-362)OE. RNA bound by MSI1 in R1 and H9, and MSI1C variants MSI1 (138-362), MSI1(272-362) were detected using RIP-seq.
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| Overall design |
RNA-seq for mESC cell line R1 (R1-WT and MSI1-KO cell line R1-C5 ), hESC cell line H9 (H9-WT, MSI1 RRM KO cell line H9-C8, MSI1 overexpression cell H9-MSI1FL and MSI1-C variant overexpression cell H9-MSI1V138 and H9-MSI1V272). RIP-seq for MSI1 binding RNA in R1 and H9 by using anti-MSI1 antibody for RIP. RIP-seq for MSI1-C variants binding RNA in H9 by using anti-Flag antibody for RIP.
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| Contributor(s) |
Lin G, Chen Y |
| Citation(s) |
37858462 |
| BioProject |
PRJNA808680 |
| |
| Submission date |
Feb 28, 2022 |
| Last update date |
Nov 06, 2023 |
| Contact name |
Gufa Lin |
| E-mail(s) |
lingufa@tongji.edu.cn
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| Organization name |
Tongji University
|
| Department |
Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Life Sciences and Technology, Tongji University
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| Street address |
1239 Siping road Tongji university
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (24)
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