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| Status |
Public on Feb 26, 2022 |
| Title |
Despite its sequence identity with canonical H4, Drosophila H4r product is enriched at specific chromatin regions |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
A single H4 variant gene (H4r) that is located outside of the histone cluster and gives rise to a polyA tailed messager RNA via replication-independent expression is preserved in Drosophila strains despite that its protein product is identical with canonical H4. In order to reveal information on the possible role of this alternative H4 we epitope tagged endogenous H4r and revealed its genome-wide localization to chromatin at the nucleosomal level. Comparing H4r-localization to the localization of another histone variant H3.3 we found that the genome-wide distribution of H4r is more similar to the histone variant H3.3 than to the canonical histone H3. Our ChIP-seq experiments revealed H4r association with regulatory regions of genes involved in cellular stress response. The data presented here indicate that H4r has a variant histone function.
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| Overall design |
Examination of the genome-wide localization of two epitope-tagged variant histones at nucleosomal level via ChIP-seq.
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| Contributor(s) |
Abraham A, Boros IM, Henn L, Villanyi Z, Zsindely N, Bodai L, Nagy G, Szabo A |
| Citation(s) |
35322122 |
| BioProject |
PRJNA789790 |
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| Submission date |
Feb 23, 2022 |
| Last update date |
Mar 25, 2022 |
| Contact name |
Andrea Ábrahám |
| Organization name |
University of Szeged
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| Street address |
Közép fasor 52.
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| City |
Szeged |
| State/province |
Csongrád |
| ZIP/Postal code |
6726 |
| Country |
Hungary |
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| Platforms (1) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (24)
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