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| Status |
Public on May 19, 2022 |
| Title |
Generation of specialized blood vessels via lymphatic transdifferentiation |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The lineage and developmental trajectory of a cell are key determinant s of cellular identity. In the vascular system, endothelial cells (ECs) of blood and lymphatic vessels (LVs) differentiate and diversify to cater the different physiological demands of each organ . While LVs are known to originate from multiple origins , lymphatic ECs (LECs) themselves are not known to generate other cell - types . Here, we u s e recurrent imaging and lineage - tracing of ECs in zebrafish anal fins (AF) from early development through adulthood , to uncover an unexpected mechanism of specialized blood vessel formation through transdifferentiation of LECs . Moreover, we demonstrate distinct functional implications for deriving AF vessels from either LECs or blood ECs, uncovering a link between cell ontogeny and functionality. We further use scRNA - seq to characterize the different cellular populations and transition states involved in the transdifferentiation process . Finally, we show that akin to its normal development, the vasculature is re - derived from lymphatics during AF regeneration, demonstr ating that LECs in adult fish retain both potency and plasticity for generating blood ECs . Overall, our work highlights a new innate mechanism of blood vess el formation through LEC trans differentiation, and provides in vivo evidence for a link between cell ontogeny and functionality in ECs
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| Overall design |
80 anal fins from immature zebrafish were digested. Sorting and RNA extraction was performed on fli1a:dsRed positive cells
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| Contributor(s) |
Das RN, Tevet Y, Safriel S, Han Y, Moshe N, Lambiase G, Bassi I, Nicenboim J, Bruckner M, Hirsch D, Eilam-Altstadter R, Herzog W, Avraham R, Poss KD, Yaniv K |
| Citation(s) |
35614218 |
| Submission date |
Feb 22, 2022 |
| Last update date |
Jun 17, 2022 |
| Contact name |
Yaara Tevet |
| E-mail(s) |
yaara.tevet@weizmann.ac.il
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| Organization name |
Weizmann Institute of Science
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| Department |
Department of Biological Regulation
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| Street address |
Hertzel 234
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| City |
Rehovot |
| State/province |
Israel |
| ZIP/Postal code |
7630031 |
| Country |
Israel |
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| Platforms (1) |
| GPL20828 |
Illumina NextSeq 500 (Danio rerio) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA809307 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197161_RAW.tar |
1.5 Mb |
(http)(custom) |
TAR (of TXT, XLSX) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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