|
| Status |
Public on Apr 28, 2022 |
| Title |
Time-course of host cell transcription during the HTLV-1 transcriptional burst |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the host cell transcription during the phases of the HTLV-1 plus-strand burst.
|
| |
|
| Overall design |
We used two naturally HTLV-1-infected clones isolated from patient blood, which were transduced with a Tax-responsive Timer construct reporter, that allowed the separation of proviral plus-strand burst into silent (DN – double negative), early (Blue), mid (DP – double positive), late phases (Red). The cells were sorted into these populations based on the Timer fluorescence and submitted to RNA sequencing. Each timepoint had two replicates.
|
| |
|
| Contributor(s) |
Bangham CR, Ramanayake S, Kiik H |
| Citation(s) |
35576236 |
| |
| Submission date |
Feb 21, 2022 |
| Last update date |
May 17, 2022 |
| Contact name |
Charles R. M. Bangham |
| E-mail(s) |
c.bangham@imperial.ac.uk
|
| Phone |
+44 20 7594 3730
|
| Organization name |
Imperial College London
|
| Department |
Infectious Disease
|
| Lab |
Bangham Lab
|
| Street address |
Norfolk Place
|
| City |
London |
| ZIP/Postal code |
W2 1PG |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA808996 |
Less...
More...