 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 28, 2023 |
| Title |
Whole blood transcriptome of five West African cattle breeds before and during an infection by Trypanosoma congolense IL1180, inference on trypanotolerance |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Animal African trypanosomosis, caused by blood protozoan parasites transmitted mainly by tsetse flies, represents a major constraint for millions of cattle in sub-Saharan Africa. Exposed cattle include West African taurine breeds called trypanotolerant according to their ability to control parasite development and to survive and grow in enzootic areas, and indicine breeds that are trypanosusceptible to the disease. Until now the genetic basis of trypanotolerance remains unclear. Here, we improved knowledge in the biological processes involved in trypanotolerance by identifying bovine genes differentially expressed during an experimental infection by Trypanosoma congolense and their biological functions. To this end, whole blood genome-wide transcriptome profiling by RNA sequencing was performed on five West African cattle breeds, three trypanotolerant taurine breeds (N'Dama, Lagune and Baoulé), one susceptible zebu (Zebu Fulani) and one African taurine x zebu admixed breed (Borgou), at four dates, one before and three during infection. As expected, infection had a major impact on cattle blood transcriptome whatever the breed. The functional analysis of differentially expressed genes over time in each breed confirmed an early activation of the innate immune response, followed by an activation of the humoral response and an inhibition of T cells functions at the chronic stage of infection. More importantly, we highlighted overlooked features, as a strong disturbance in host metabolism and cell production energy that differentiate trypantolerant and trypanosusceptible breeds. N'Dama breed showed the earliest regulation of immune response, associated with a strong activation of cellular energy production, this last feature being also shared with Lagune, and to a lesser extent with Baoulé. Susceptible Zebu Fulani breed was distinguished from other breeds by the strongest modification in lipid metabolism regulation. Lastly, basal differences in gene expression reflected the structuration of cattle genetic diversity, and could have consequences on the tolerant or susceptible phenotype. Overall, it would be of value to deeper investigate interactions between immune response and cell metabolism that likely impact disease outcome.
|
| |
|
| Overall design |
A total of 120 RNA-seq libraries were generated from 30 cattle from five West African cattle breeds (6 animals per breed, all males, between 1 year and 2.5 years old). The breeds were: N'Dama (NDA) from Mali, Lagune (LAG) from Benin, Baoulé (BAO) from Burkina Faso, Borgou (BOR) from Benin, and Fulani Zebu (ZFU) from Burkina Faso. These animals were experimentally infected by Trypanosoma congolense IL1180. Blood samples of the 30 animals were collected at one date before infection (0), and at three dates after infection: at 20, 30 and 40 days post-infection (DPI). RNA was extracted from the 30 cattle at these four dates and sequenced to produce 120 RNA-seq libraries. The libraries were equimolarly pooled by 12, leading to 10 multiplexes of 12 samples each. Phenotypic analysis was published in Berthier et al 2015 "A Comparison of Phenotypic Traits Related to Trypanotolerance in Five West African Cattle Breeds Highlights the Value of Shorthorn Taurine Breeds." PLoS One 10, no. 5 (2015): e0126498. DOI: 10.1371/journal.pone.0126498. SNP genotypes using the Illumina BovineSNP50.v2 SNP are freely available in WIDDE DB, project AATTOL: http://widde.toulouse.inra.fr/widde/widde/main.do?module=cattle.
|
| |
|
| Contributor(s) |
Thévenon S, Berthier D, Peylhard M, Flori L, Dayo G, Chantal I, Martin G, Sempéré G, Sylla S, Nidelet S, Dubois E |
| Citation |
Whole blood transcriptome profiles of trypanotolerant and trypanosusceptible cattle highlight a differential modulation of metabolism and immune response during infection by Trypanosoma congolense. Peylhard M., Berthier D., Dayo G-K., Chantal I., Sylla S., Nidelet S., Dubois E., Martin G., Sempé, Flori L., Thé S. Peer Community Journal, 2023, Volume 3, article no. e17.
|
| |
| Submission date |
Feb 21, 2022 |
| Last update date |
Feb 28, 2023 |
| Contact name |
Sophie Thévenon |
| E-mail(s) |
sophie.thevenon@cirad.fr
|
| Organization name |
CIRAD
|
| Department |
BIOS
|
| Lab |
UMR INTERTRYP
|
| Street address |
TA A-17/G Campus International de Baillarguet
|
| City |
MONTPELLIER |
| ZIP/Postal code |
34398 CEDEX 5 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL15749 |
Illumina HiSeq 2000 (Bos taurus) |
|
| Samples (120)
|
|
| Relations |
| BioProject |
PRJNA808995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197108_AATTOL_counts_STAR_120_unik.txt.gz |
5.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...