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Series GSE19693 Query DataSets for GSE19693
Status Public on Dec 30, 2009
Title STAR RNA-binding protein, Quaking, suppresses cancer via regulation of microRNA
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Human polyomavirus 1; Macaca mulatta polyomavirus 1
Sample organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary MicroRNAs have emerged as major genetic elements in the genesis and suppression of cancer. Here, multi-dimensional cancer genome analysis and validation has defined a novel Glioblastoma Multiforme (GBM) tumor suppressor pathway and mechanism of action centered on Quaking (QK), a member of the STAR family of RNA-binding proteins. Combined functional, biochemical and computational studies establish that p53 directly regulates QK gene expression, QK protein binds and stabilizes miR-20a of the cancer-relevant miR-17-92 cluster, and miR-20a in turn functions to regulate TGFβR2 and the TGFβ signaling network. Linkage of these pathway components is supported by their genome and expression status across GBM specimens and by their gain- and loss-of-function interactions in in vitro and in vivo complementation studies. This p53-QK-miR-20a axis expands our understanding of the p53 tumor suppression network in cancer and reveals a novel tumor suppression mechanism involving regulation of specific cancer-relevant microRNAs.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series.
 
Citation(s) 22751500
Submission date Dec 28, 2009
Last update date Feb 11, 2019
Contact name Hailei Zhang
E-mail hailei_zhang@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Street address 44 Binney Street
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (3)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL7723 miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno
Samples (48)
GSM491519 Hs683_2a
GSM491520 Hs683_2b
GSM491521 Hs683_2c
This SuperSeries is composed of the following SubSeries:
GSE19686 shGFP- and shQK-transduced human Hs683 cells
GSE19687 shGFP- and shQk-transduced Ink4a/Arf-/- Pten-/- primary mouse astrocytes
GSE19688 QK-knockdown Hs683 transduced with miR-20a or a scrambled non-targeting microRNA (miR-NT)
Relations
BioProject PRJNA121925

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19693_RAW.tar 149.0 Mb (http)(custom) TAR (of CEL, TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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