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Series GSE19668 Query DataSets for GSE19668
Status Public on Aug 01, 2010
Title Genetic Determinants for Susceptibility to Staphylococcus aureus Infection in A/J and C57BL/6J
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although it has recently been shown that A/J mice are highly susceptible to Staphylococcus aureus sepsis as compared to C57BL/6J, the specific genes responsible for this differential phenotype are unknown. Using chromosome substitution strains (CSS), we found that factors on chromosomes (chr) 8, 11, and 18 are responsible for susceptibility to S. aureus sepsis in A/J mice. F1 mice from C57BL/6J X CSS8 cross (C8A) and C57BL/6J X CSS18 (C18A) were also susceptible to S. aureus (median survival < 48 h), whereas F1 mice from C57BL/6J X CSS11 cross (C11A) were resistant (median survival > 120 h) to S. aureus. Bacterial loads in the kidney were consistent with F1 median survivals, with higher bacterial counts in susceptible mice. No sexlinked associations with susceptibility were noted in F1 intercrosses. Using whole genome transcription profiling, we identified a total of 192 genes on chromosomes 8, 11, and 18 which are differentially expressed between A/J and C57BL/6J in the setting of S. aureus infection. Of these, 28 genes had Gene Ontology annotations indicating a potential immune response function. These 28 genes are associated with susceptibility to S. aureus in A/J mice, and are potential determinants of susceptibility to S. aureus infection in humans.
 
Overall design To identify genes for which differential expression between A/J and C57BL/6J mice could contribute to host susceptibility to S. aureus infection, we compared the gene expression profiles between uninfected A/J and C57BL/6J mice and between infected A/J and C57BL/6J mice at 2, 4, 6, and 12 hours after infection.
 
Contributor(s) Ahn S, Cowell LG
Citation(s) 20824097
Submission date Dec 28, 2009
Last update date Feb 11, 2019
Contact name Lindsay Cowell
E-mail lgcowell@duke.edu
Organization name Duke University
Street address 2424 Erwin Road
City Durham
State/province NC
ZIP/Postal code 27705
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (50)
GSM491049 blood A/J 0hours rep5
GSM491050 blood A/J 2hours rep5
GSM491051 blood A/J 4hours rep5
Relations
BioProject PRJNA122487

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Supplementary file Size Download File type/resource
GSE19668_RAW.tar 171.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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