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| Status |
Public on Feb 10, 2023 |
| Title |
Thrombin exerts an inflammatory role in chondrocytes through activating the MEK-Erk1/2-Stat3 signaling pathway. |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Rat chondrocytes were divided into control group (RP), thrombin group (RPT), and thrombin plus inhibitor group (RPTi). The differential genes were identified by RNA-sequencing, and verified by western blot (WB), quantitative polymerase chain reaction (qPCR), cell immunofluorescence (IF), endoplasmic reticulum (ER) staining, functional enrichment analysis, and Gene Ontology (GO).
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| Overall design |
To study the mechanism of thrombin regulating chondrocytes function based on RNA-sequencing and experimental verification.
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| Contributor(s) |
Ding D |
| Citation missing |
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| |
| Submission date |
Feb 10, 2022 |
| Last update date |
Feb 10, 2023 |
| Contact name |
daofang ding |
| E-mail(s) |
dingdaofang@shutcm.edu.cn
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| Organization name |
Shanghai University of Traditional Chinese Medicine
|
| Street address |
1200 Cai Lun Road,Zhangjiang Hi-TechPark,Pudong New Area
|
| City |
shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
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| Platforms (1) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA805215 |
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