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Series GSE19639 Query DataSets for GSE19639
Status Public on Jun 15, 2010
Title Hyperactivation of PI3K promotes escape from hormone dependence in estrogen receptor-positive breast cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer.

A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged.
 
Overall design Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.
 
Contributor(s) Miller T, Arteaga C
Citation(s) 20530877, 21346144
Submission date Dec 23, 2009
Last update date Mar 25, 2019
Contact name Todd W Miller
E-mail(s) todd.miller@vanderbilt.edu
Organization name Vanderbilt Univ
Department Medicine
Street address 2220 Pierce Ave, 777 PRB
City Nashville
State/province TN
ZIP/Postal code 37174
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (24)
GSM490043 MCF-7, rep 1
GSM490044 MCF-7, rep 2
GSM490045 MCF-7, rep 3
Relations
BioProject PRJNA122527

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19639_RAW.tar 116.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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