|
| Status |
Public on Jan 19, 2023 |
| Title |
Characterization of maturational enhancer activity by massively parallel reporter assay [amplicon-Seq] |
| Organisms |
Mus musculus; Adeno-associated virus 9 |
| Experiment type |
Other
|
| Summary |
The activity of enhancers with dynamic P300 binding or mutagenized nuclear receptor motifs was assessed by massively parallel reporter assay during CM maturation.
|
| |
|
| Overall design |
AAV9 reporter pools were injected at P0 or P7 and tissue was harvested for measurement of enhancer activity at P0, P7, or P28 by amplicon sequencing.
|
| |
|
| Contributor(s) |
VanDusen NJ, Pu WT |
| Citation(s) |
37071996 |
| |
| Submission date |
Feb 08, 2022 |
| Last update date |
Apr 20, 2023 |
| Contact name |
Pingzhu Zhou |
| E-mail(s) |
ping-zhu.zhou@childrens.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Department |
Cardiology
|
| Lab |
William Pu
|
| Street address |
320 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL31917 |
Illumina NextSeq 500 (Adeno-associated virus 9) |
|
| Samples (88)
|
|
| This SubSeries is part of SuperSeries: |
| GSE195905 |
Characterization of dynamic p300 enhancers in murine cardiomyocyte development |
|
| Relations |
| BioProject |
PRJNA804494 |
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