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| Status |
Public on Apr 26, 2024 |
| Title |
Cholesterol in macrophage regulate gene expersion with or without LPS [statin and LPS RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
cholesterol reduction in macrophages by methyl-b-cyclodextran (MCD) or statin alters gene expression. When activated by inflammatory stimilus LPS, macrophages responded in a cholesterol specific fashion.
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| Overall design |
RNA-seq was performed on rest RAW macrophages, treated with MCD or statin. Similar RNA-seq also performed on macropahges stimulated with LPS. Two replicates were performed for untreated control (ctl),; two replicates for MCD and statin treated cells (MCD and Statin), respectively. Control cells were stimulated with LPS (ctl-LPS), two replicates, and also statin treated cells were stimulated LPS identically (statin-LPS).
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| |
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| Contributor(s) |
Dauner K, Zha X |
| Citation(s) |
38602170 |
| |
| Submission date |
Feb 05, 2022 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Jeffrey Dilworth |
| E-mail(s) |
fdilworth@wisc.edu
|
| Phone |
6082653758
|
| Organization name |
University of Wisconsin
|
| Street address |
1111 Highland Ave
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA803745 |
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