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Series GSE19557 Query DataSets for GSE19557
Status Public on Jan 07, 2010
Title miRNA expression in C. elegans fem-2(b245ts) and fem-3(q20ts) mutants
Organism Caenorhabditis elegans
Experiment type Non-coding RNA profiling by array
Summary We compared animals that produce oocytes but no sperm fem-2(b245ts) to animals that produce sperm but no oocytes fem-3(q20ts). Using miRNA microarrays and we found that the mir-35-41 cluster genes were expressed to much greater extent in oogenesis than in spermatogenesis; furthermore, these miRNAs were the only C. elegans miRNAs that showed such differential germline expression.
Overall design Total RNA was isolated from staged one day old adult populations. The data were obtained from two biological replicates. Each oligonucleotide was printed four times. Data were scaled based on total array intensities (sum of the normalized spot intensities), and data for each sample and each gene were averaged.
Contributor(s) Alvarez-Saavedra E, Horvitz HR
Citation(s) 20096582
Submission date Dec 18, 2009
Last update date Mar 21, 2012
Contact name Ezequiel Alvarez-Saavedra
Organization name MIT
Department Biology
Lab Horvitz
Street address 77 Massachusetts Avenue Room 68-411
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL9815 MIT-HRH-Celegans-MAS8
Samples (6)
GSM487566 fem-2(b245ts)-1day old adults at 25C-technicalrep1-biolrep1
GSM487567 fem-2(b245ts)-1day old adults at 25C-technicalrep2-biolrep1
GSM487568 fem-2(b245ts)-1day old adults at 25C-technicalrep1-biolrep2
BioProject PRJNA122399

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Supplementary file Size Download File type/resource
GSE19557_RAW.tar 190.0 Kb (http)(custom) TAR (of CSV)
Processed data included within Sample table

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