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| Status |
Public on Jan 30, 2022 |
| Title |
Hierarchical deployment of Tbx3 dictating the identity of hypothalamic Tac2/Kiss1 neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
To investigate how Tbx3 regulates the fate determination of arcuate piptidergic neruons, we performed scRNA-seq, snRNA-seq and CUT&Tag to reveal the function of Tbx3 in fate specification and maintenance of neurons
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| Overall design |
Lineage tracing experiment: scRNA-seq of Tbx3-CreER::Ai14 mice (E9 > P14); Tbx3 knockout experiment: scRNA-seq and snRNA-seq of Nkx2.1-Cre::Tbx3 (Flox/Flox) mice and its littermate control (P14); CUT&Tag experiment: Nkx2.1-Cre::Tbx3 (Flox/Flox) mice and its littermate control (P0)
Please note that NKO mice is a conditional knockout mice strain that lacking the expression of Tbx3 in hypothalamus and, like the input sample, peak-calling was performed based on reads of the blank control (NKO mice) in the experiment of CUT&Tag. Therefore no processed data was provided for both 'NKO mice_CUT&Tag_WT_input' and 'NKO mice_CUT&Tag_NKO_Ab' samples.
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| Contributor(s) |
Shi X, Wu Q |
| Citation(s) |
36383654 |
| |
| Submission date |
Jan 26, 2022 |
| Last update date |
Dec 15, 2022 |
| Contact name |
Qing-Feng Wu |
| E-mail(s) |
wu_qingfeng@genetics.ac.cn
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| Organization name |
Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
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| Street address |
No. 1 West Beichen Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (2) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA800757 |
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