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Series GSE19540 Query DataSets for GSE19540
Status Public on Dec 21, 2009
Title microRNA expression in rat lung receiving chemopreventive agents and attenuation of cigarette smoke-induced alterations
Platform organisms Arabidopsis thaliana; Homo sapiens; Mus musculus
Sample organism Rattus norvegicus
Experiment type Non-coding RNA profiling by array
Summary We previously showed that exposure of rats to environmental cigarette smoke (ECS) causes extensive downregulation of microRNA expression in the lung, resulting in overexpression of multiple genes and proteins. In the present study, we evaluated by microarray the expression of 484 microRNAs in the lung of rats receiving orally chemopreventive agents, including N-acetylcysteine, oltipraz, indole-3-carbinol, 5,6-benzoflavone and phenethyl isothiocyanate, or combinations thereof. Scatterplot, hierarchical cluster, and principal component analyses showed that none of the above chemopreventive regimens appreciably affected the baseline microRNA expression, while all of them attenuated ECS-induced alterations but to a variable extent. Thus, mirnome analysis provides a new tool for predicting both safety and efficacy of cancer chemopreventive agents at early carcinogenesis stages.

Keywords: cancer chemoprevention, microRNA, environmental cigarete smoke (ECS), 5,6-benzoflavone (BF), Indole 3-carbinol (I3C),N-acetylcysteine (NAC), oltipraz (OPZ), Phenethyl isothiocyanate (PEITC).
Overall design Adult male Sprague-Dawley rats (Harlan Italy, Correzzana, Milan, Italy), weighing 315-320 g, were divided into 16 groups, each composed of 8 animals. Half of them were exposed to ECS for 28 consecutive days, as previously described (Izzotti et al., 2005), while the remaining rats (Sham-exposed) were kept for the same period of time in filtered air. The rats belonging to 14 groups were treated with chemopreventive agents, starting 3 days before exposure to ECS.
Contributor(s) Izzotti A, Calin GA, Steele VE, Cartiglia C, Longobardi M, Croce CM, De Flora S
Citation(s) 20051373
Submission date Dec 17, 2009
Last update date Mar 21, 2012
Contact name Alberto Izzotti
Phone 010 3538394
Fax 010 3538504
Organization name University of Genoa
Department Dept. of Health Sciences
Street address A. Pastore, 1
City Genoa
ZIP/Postal code 16132
Country Italy
Platforms (1)
Samples (15)
GSM487321 Lung Control
GSM487322 Lung ECS
GSM487323 Lung PEITC
BioProject PRJNA122317

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Supplementary file Size Download File type/resource
GSE19540_RAW.tar 6.7 Mb (http)(custom) TAR (of GPR)
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Raw data provided as supplementary file
Processed data included within Sample table

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