|
| Status |
Public on Dec 01, 2010 |
| Title |
A rapid and scalable system for studying gene function in mice using conditional RNA interference |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luciferase miRNA is expressed as the 8th most abundant species in the cells.
Keyword(s): Epigenetics
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| |
|
| Overall design |
4 libraries were analyzed
|
| |
|
| Contributor(s) |
Premsrirut PK, Malone CD, Lowe SW |
| Citation(s) |
21458673 |
| Submission date |
Dec 15, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Colin Dean Malone |
| E-mail(s) |
colin.malone@med.nyu.edu
|
| Phone |
708-602-2170
|
| Organization name |
NYU Medical Center
|
| Department |
Skirball Institute - Developmental Genetics
|
| Lab |
Lehmann Lab
|
| Street address |
NYUMC, Skirball Institute, 540 1st Ave, 4-10
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (4)
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|
| Relations |
| SRA |
SRP002217 |
| BioProject |
PRJNA122263 |
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