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Series GSE19446 Query DataSets for GSE19446
Status Public on Mar 21, 2010
Title Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers
Organism Mus musculus
Experiment type Expression profiling by array
Summary To delineate epithelial subpopulations in mouse mammary tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from mammary glands using fluorescence-activated cell sorting. The resultant Lin- population was fractionated into four distinct subpopulations using antibodies against CD29, CD24 and CD61. Based on the immunohistochemical phenotype, and in vivo and in vitro functional assays, these subpopulations were identified as fibroblast-containing stromal (CD29loCD24-), mammary stem cell (MaSC)-enriched (CD29hiCD24+CD61+), luminal progenitor (CD29loCD24+CD61+), and mature luminal (CD29loCD24+CD61-) cell subpopulations. Microarray profiling was used to derive gene expression signatures of these 4 subpopulations. The four mammary cell subpopulations were found to have distinct gene expression profiles.
 
Overall design Four mammary cell subpopulations from 3-5 pooled mouse tissues were analysed. MS is for the MaSC-enriched cell subpopulation. LP is for the Luminal Progenitor subpopulation. ML is for the Mature Luminal subpopulation.
 
Contributor(s) Smyth GK, Wu D, Lim E, Visvader JE, Lindeman GJ
Citation(s) 20346151
Submission date Dec 13, 2009
Last update date Jan 16, 2019
Contact name Jane E Visvader
E-mail visvader@wehi.edu.au
Phone (+61 3) 93452494
Fax (+61 3) 93470852
Organization name The Walter and Eliza Hall Institute of Medical Research
Department Victorian Breast Cancer Research Consortium
Lab Lindeman/Visvader Lab
Street address 1G Royal Parade
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (18)
GSM484660 stroma, replicate 1
GSM484661 ML, replicate 1
GSM484662 LP, replicate 1
Relations
BioProject PRJNA121805

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19446_RAW.tar 15.8 Mb (http)(custom) TAR
GSE19446_non-normalized_data.txt.gz 3.5 Mb (ftp)(http) TXT
Raw data is available on Series record
Processed data included within Sample table

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