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Series GSE194399 Query DataSets for GSE194399
Status Public on Feb 11, 2022
Title Obesity-induced senescent macrophages activate a fibrotic transcriptional program in adipocyte progenitors
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adipose tissue fibrosis is regulated by the chronic and progressive metabolic imbalance caused by differences in caloric intake and energy expenditure. By exploring the cellular heterogeneity within fibrotic adipose tissue, we demonstrate that early adipocyte progenitor cells expressing both platelet derived growth factor receptor (PDGFR) α and β are the major contributors to extracellular matrix deposition. We show that the fibrotic program is promoted by senescent macrophages. These macrophages were enriched in the fibrotic stroma and exhibit a distinct expression profile. Furthermore, we demonstrate that these cells display a blunted phagocytotic capacity and acquire a senescence-associated secretory phenotype. Finally, we determined that osteopontin, which was expressed by senescent macrophages in the fibrotic environment promoted progenitor cell proliferation, fibrotic gene expression, and inhibited adipogenesis. Our work reveals that obesity promotes macrophage senescence and provides a conceptual framework for the discovery of rational therapeutic targets for metabolic and inflammatory disease associated with obesity.
Overall design Single cell of the stromal vascular fraction from mice epididymal adipose tissue following 24 weeks of either high fat or low fat diet feeding
Contributor(s) Rabhi N, Desevin K, Belkina AC, Desevin K, Tilston-Lunel A, Varelas X, Layne MD, Farmer SR
Citation(s) 35181634
Submission date Jan 25, 2022
Last update date Mar 15, 2022
Contact name Nabil Rabhi
Phone 8572659274
Organization name Boston University
Department Biochemistry
Lab Farmer Lab
Street address Silvio Conte Building, 72 E. Concord St
City Boston
State/province Massachusetts
ZIP/Postal code 02118
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM5835151 e_LFD
GSM5835152 e_HFD
BioProject PRJNA800421

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Supplementary file Size Download File type/resource
GSE194399_RAW.tar 17.5 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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