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Series GSE194390 Query DataSets for GSE194390
Status Public on Feb 14, 2022
Title Angioplasty induces epigenomic remodeling in injured arteries
Organism Rattus norvegicus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Neointimal hyperplasia (IH) is a primary etiology of stenotic vascular diseases. It is perpetuated principally by smooth muscle cell proliferation. Epigenome-scale studies concerning IH have been confined to in vitro models, and the IH-underlying epigenetic mechanisms remain poorly understood. The current study has integrated information from in vivo epigenomic mapping, conditional knockout, gene transfer and pharmacology in rodent models of IH. The data from injured (IH-prone) rat arteries revealed a surge of genomewide occupancy by H3K27me3, a gene repression mark. This was unexpected in the traditional view of prevailing post-injury gene activation rather than repression. Further analysis illustrated a shift of H3K27me3 enrichment to anti-proliferative genes, from pro-proliferative genes where gene activation mark H3K27ac instead accumulated. In accordance, H3K27ac and its reader BRD4 co-enriched at Ezh2 governing its expression. Moreover, H3K27me3 writer EZH2 positively regulated another recently identified pro-IH chromatin modulator UHRF1. Thus, results unravel injury-induced loci-specific H3K27me3 redistribution in the epigenomic landscape entailing BRD4->EZH2->UHRF1 hierarchical regulations, and may guide translation to treat IH given that these players are pharmaceutical targets.
Overall design ChIPseq was performed on injured and uninjured rats for 4 histone markers.
Contributor(s) Guo LW, Webb A, Gulcin Ozer H
Citation(s) 35169042
Submission date Jan 25, 2022
Last update date Mar 15, 2022
Contact name Amy Hite
Organization name The Ohio State University
Department Biomedical Informatics
Street address 1800 Cannon Drive 250 Lincoln Tower
City Columbus
State/province OH - Ohio
ZIP/Postal code 43210
Country USA
Platforms (1)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
Samples (9)
GSM5834952 1_Injured_BRD4
GSM5834953 2_Uninjured_BRD4
GSM5834954 3_Injured_H3K27Ac
BioProject PRJNA800429

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE194390_0079Wis_SICER_intervals.xlsx 18.9 Mb (ftp)(http) XLSX
GSE194390_RAW.tar 6.6 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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