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Series GSE193816 Query DataSets for GSE193816
Status Public on Feb 24, 2023
Title A model of human asthma exacerbation identifies novel transcriptional programs and pathogenic cell circuits in the lower airway mucosa specific to allergic asthma
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Using a human model of asthma exacerbation, we compared the airway mucosa in allergic asthmatics and allergic non-asthmatic controls using single-cell RNA-sequencing frameworks. In response to allergen challenge, the airway epithelium in asthmatics was highly dynamic and upregulated genes involved in matrix degradation, mucus metaplasia, and glycolysis while failing to induce injury-repair and antioxidant pathways observed in controls. Asthmatics also had a unique mucosal immune profile, characterized by IL9-expressing pathogenic TH2 cells and enrichment of DC2 (CD1C) and CCR2-expressing monocyte-derived cells (MC) after allergen, with upregulation of genes that promote pathologic airway remodeling. In contrast, controls were enriched for macrophage-like MC that upregulated tissue repair programs after allergen challenge, suggesting these populations may protect against asthmatic airway remodeling. These findings reveal a novel TH2-mononuclear phagocyte-epithelial interactome unique to asthmatics, suggesting that pathogenic effector circuits and the absence of pro-resolution programs drive structural airway disease in response to type 2 inflammation.
 
Overall design scRNAseq of lung cells captured by endobronchial brushes in allergic asthmatics (n = 4) and allergic controls (n = 4) under 3 conditions (baseline, diluent (saline), an allergen).
 
Contributor(s) Smith NP
Citation(s) 37146132
Submission date Jan 17, 2022
Last update date Oct 13, 2023
Contact name Neal Patrick Smith
E-mail(s) nsmith19@mgh.harvard.edu
Organization name Massachusetts General Hospital
Street address 149 13th Street
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (21)
GSM5821830 500008_ANA_Ag
GSM5821831 500008_ANA_Dil
GSM5821832 500008_ANA_Pre
Relations
BioProject PRJNA798033

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193816_AEC_data.h5ad.gz 222.1 Mb (ftp)(http) H5AD
GSE193816_all_cells_data.h5ad.gz 532.5 Mb (ftp)(http) H5AD
GSE193816_all_data_log_adjusted_counts.h5ad.gz 217.4 Mb (ftp)(http) H5AD
GSE193816_all_data_raw_counts.h5ad.gz 214.3 Mb (ftp)(http) H5AD
GSE193816_mnp_data.h5ad.gz 108.4 Mb (ftp)(http) H5AD
GSE193816_t_cell_data.h5ad.gz 142.5 Mb (ftp)(http) H5AD
Raw data not provided for this record
Processed data are available on Series record

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