|
| Status |
Public on Feb 10, 2010 |
| Title |
Regulation of alternative splicing by histone modifications |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Alternative splicing of pre-mRNA is a prominent mechanism to generate protein diversity, yet its regulation is poorly understood. Here, we demonstrate a direct role for histone modifications in alternative splicing. We find distinctive histone modification signatures which correlate with splicing outcome in a set of human genes. Modulation of histone modifications causes splice site switching. The mechanism for histone-mediated splice site selection involves a histone mark which is read by a chromatin protein, which in turn recruits a splicing regulator. These results outline an adaptor system for reading of histone marks by the pre-mRNA splicing machinery.
|
| |
|
| Overall design |
To obtain an estimate of how many PTB-dependent alternative splicing events are regulated by SET2/MRG15-mediated recruitment of PTB, we carried out a genomewide comparative analysis of alternative splicing in hMSC cells depleted of either SETD2, MRG15 or PTB using specific siRNAs, or mock-depleted using a control siRNA.
|
| |
|
| Contributor(s) |
Luco RF, Pan Q, Tominaga K, Blencowe BJ, Pereira-Smith OM, Misteli T |
| Citation(s) |
20133523 |
| |
| Submission date |
Dec 08, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Qun Pan |
| E-mail(s) |
qun.pan@utoronto.ca
|
| Organization name |
University of Toronto
|
| Street address |
160 College St.
|
| City |
Toronto |
| ZIP/Postal code |
M5S 3E1 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| SRA |
SRP001893 |
| BioProject |
PRJNA120419 |
Less...
More...